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Measurement of Lipid Peroxidation Lipid peroxidation level in the brain cell homogenate was quantified using the thiobarbituric acid reactive substances (TBARS) as previously described (Buege and Aust, Measurement of Protein Carbonyl Content Protein carbonyls were used as a measure of protein oxidation, in brain cell homogenates using DNPH as previously described (Levine et al., Measurement of Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) of the Antioxidant Enzymes Activity Superoxide dismutase (SOD) and GPx activities were quantified using commercially available kits (Cayman chemicals) according to the manufacturers guidelines
This excess hydrogen peroxide essentially "bleaches" the hair from the inside out by damaging the melanocytes and interfering with the production of melanin
H Representative transmission electron microscopy (TEM) images
Exclusion criteria included animal studies
It also promoted the expression of Arg-1, IL-10, and TGF-, inhibiting ferroptosis and inducing BV2 cell polarization toward the M2 phenotype, thereby mitigating BV2 cell injury
